By Vipin K Agarwal; American Chemical Society. Division of Agricultural and Food Chemistry
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Additional resources for Analysis of antibiotic/drug residues in food products of animal origin
G. the CFT. In some cases weak binding of the antibiotic to the receptor limits the MRA sensitivity. When this happens an antibody binder may be better, especially in a drug family with few members. In the CTII, chloramphenicol and tetracyclines use antibody binders. 35 e SULFA DRUGS MACROLIDES NOVOBIOCIN MICROBIAL CELL Charm Test Receptor Binding - Antimicrobial drugs in the sample compete with [14C] or [3H] labeled antimicrobials for specific sites in the microbial cell. T =tetracycline P = penicillin p14 = [14C] penicillin T3 = [3H] tetracycline * Although tetracyclines and chloramphenicol may be detected with a receptor assay, antibody binding Is found to be superior in these assays, and has been incorporated in the Charm Test.
Medicated feed has the characteristic of producing wide spread contamination. It is legally possible to feed 140 mg/day/head (1400 cow), for 30 days, according to Code of Federal Regulations (CFR), (22). It is not clear what this really means since how much time should elapse between such feeding is not specified. In comparing the HPLC-Receptorgram for CTC in feed, and the CTC residue in milk, a conversion of CTC (feed) to tetracycline (T) in milk is observed (see Figure 5). The feed contains a ratio of CTC to T of 20:1 while the derived milk contains 4:1.
REPERENCES 1. T. W. A. E. E. Barnett. C. A. " J. of Food Protection, Vol 52, No. 12, Pages 867-870, (December 1989). 2. R. E. M. D. Whittier, 1991. " Report from Virginia Polytechnic Institute and State University, Blacksburg, VA 24061. Delivered at the NCIMS Meeting April 1991, Louisville, KY. 3. , 36 Franklin Street, Malden, MA 02148. 4. E. K Ruey, 1982. " J. Assoc. , Malden, MA). 5. E. Charm, 1980. " U. S. Patent 4,238,521. 6. E. K. Ruey, 1988. " J. Assoc. , P304-316. 7. E. Charm, E. Zomer, and R.
Analysis of antibiotic/drug residues in food products of animal origin by Vipin K Agarwal; American Chemical Society. Division of Agricultural and Food Chemistry