By A H Rebar; et al
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Additional resources for A Guide to Hematology in Dogs and Cats
Scratches are seen microscopically as linear disruptions of cells, particularly red cells. ® Cells broken in this manner may be mistaken for schistocytes. Pseudothrombocytopenia ® Clumping of platelets can falsely decrease the platelet counts especially in cats (Figure 4-23). 41 TNM GuideToHema:03058 TNM text_cp 6/28/07 3:49 PM Page 42 Figure 4-20 Canine blood. Granular artifact. Stain precipitate can sometimes adhere to RBCs and produce the appearance of a parasite or inclusion. These granules vary in size and do not resemble any of the usual canine RBC parasites.
Autoagglutination on blood films is seen as three-dimensional clumping of erythrocytes. This may be confused with rouleaux formation. ® Autoagglutination can be distinguished from rouleaux by preparing and examining a saline-diluted wet preparation. – Place 1 drop of EDTA-blood on a slide. – Add 2 drops of isotonic saline. – Coverslip and examine. If clumping is present, autoagglutination is confirmed. Hemolytic Disease: Heinz Body Anemia ® Occurs in dogs and cats ® Pathogenesis: – Circulating oxidants act on red cell hemoglobin at two primary sites: the sulfhydryl containing amino acids in globin, and the iron moiety.
Autoagglutination can be distinguished from rouleaux by preparing and examining a saline-diluted wet preparation. – Place 1 drop of EDTA-blood on a slide. – Add 2 drops of isotonic saline. – Coverslip and examine. If clumping is present, autoagglutination is confirmed. Hemolytic Disease: Heinz Body Anemia ® Occurs in dogs and cats ® Pathogenesis: – Circulating oxidants act on red cell hemoglobin at two primary sites: the sulfhydryl containing amino acids in globin, and the iron moiety. – Oxidation of globin leads to precipitation and the formation of Heinz bodies.
A Guide to Hematology in Dogs and Cats by A H Rebar; et al